Mitochondrial Function Imaging Analysis
Mitochondria are organelles called cell power chambers, which participate in many other cell activities, such as lipid modification, redox balance, calcium balance, and even control cell death. Therefore, imaging analysis of the mitochondrial function and shape changes of liver cells is of great significance for the evaluation of drug toxicity and help to save cost in the stage of drug development.
Figure 1. Mitochondrial imaging (Glancy B, 2020).
Mitochondrial Function Imaging Analysis
CD BioSciences uses the high-content imaging system to image and analyze the mitochondrial function of liver cells to further understand the mechanism of drug induced liver injury. The destruction of mitochondrial function can be assessed by changes in mitochondrial calcium, superoxide, mitochondrial permeability transition and membrane potential. The change of membrane potential is a good indicator of mitochondrial dysfunction, and it is increasingly related to drug toxicity.
| Analysis method | High content imaging |
| Analysis instrument | High content imaging system |
| Cell models | HepaRG, HepG2, primary human hepatocytes, with or without NPCs Other cells upon request |
| Markers | Membrane potential, superoxide production, calcium, mitochondrial permeability transition |
| Sample requirements | 50µL of 20 mM solution or equivalent amount of solid |
Liver Fibrosis Imaging Analysis Workflow
The 2D in vitro model derived from human cells may lack transporters and enzymes that promote drug metabolism and bile acid secretion. Therefore, 3D cell culture models are increasingly used in the study of drug-induced liver injury. We used the 3D model of the liver to study the mitochondrial function of liver cells. The detailed steps are as follows:
Step 1
Culture cells to produce hepatic spheroids.
Step 2
The diameter of the liver sphere grows to about 200 microns.
Step 3
Treat with test compound.
Step 4
Liver spheroids are marked with stain and fixed.
Step 5
Tissue removal makes the liver spheroid transparent and performs high-content imaging.
Step 6
Analyze images to evaluate mitochondrial function.
Delivery
Total cell counts
Viable and non-viable cell counts
Our Advantages
- Multiple measurement endpoints can be used to assess mitochondrial function
- Fully automatic high-speed microscopic imaging generates a large number of images in a short time
- Convert biological images into statistically significant quantitative data
- Professional technicians provide you with high-quality personalized services
- Reduced workload and cost
CD BioSciences has a professional team and advanced high content imaging equipment. We can provide you with imaging analysis services of liver cells mitochondrial function by high-content imaging. According to your needs, we will design a personalized analysis plan for you to ensure a better completion of your project. If you have any needs for liver cells' mitochondrial function imaging analysis, please feel free to contact us.
- Glancy B. Visualizing mitochondrial form and function within the cell[J]. Trends in molecular medicine, 2020, 26(1): 58-70.
- Mitra K, Lippincott‐Schwartz J. Analysis of mitochondrial dynamics and functions using imaging approaches[J]. Current protocols in cell biology, 2010, 46(1): 4.25. 1-4.25. 21.
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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!