Mitochondrial Membrane Potential Imaging Services
Mitochondrial membrane potential imaging is a key technique for studying mitochondrial function. It allows researchers to observe and record small changes in the mitochondrial membrane potential (MMP), which is formed by unevenly distributed ions within the mitochondrial membrane. Maintaining a normal MMP (typically -180 mV to -200 mV) is crucial for proper mitochondrial function. Depolarization of the MMP indicates impaired mitochondrial function, which is associated with cellular balance, drug toxicity, and apoptosis. By using mitochondrial membrane potential imaging, CD Biosciences can help our customers monitor real-time MMP changes, which may significantly impact mitochondrial function and disease progression.
Fig. 1 The mitochondrial membrane potential (MMP) (Zorova LD, et al. 2018).
Mitochondrial Membrane Potential Imaging Service
CD BioSciences provides the mitochondrial membrane potential imaging service for intuitive and accurate study of mitochondrial function that allows for real-time monitoring of transient and long-term changes in MMP in live cells. In addition, we can use cell labeling, apoptosis, or cytotoxicity reagents in combination to further examine cell health. Our services mainly cover the following, but are not limited to:
Continuously track MMP changes
- Track and analyze changes in MMPs over time, providing insights into cellular homeostasis and disease progression.
- Helping clients better understand cellular health and mitochondrial dysfunction associated with pathologies such as diabetes, cancer, and neurodegenerative diseases.
Kinetic measurements of mitochondrial function
Detect mitochondrial stress and obtain kinetic measurements of mitochondrial function to assess mitochondrial health and guide drug screening and optimization of treatment strategies.
Automated quantitative measurement of MMP
Utilize automated, quantitative, and reproducible methods to measure changes in mitochondrial membrane potential in living cells, ensuring the accuracy and reliability of data analysis.
Visual analysis of cell morphological changes
Visualize and analyze changes of cell morphology over time using multiple assays and cell health reagents. This helps to obtain cellular health status related to metabolic changes, including apoptosis or cytotoxicity readouts.
Mitochondrial Membrane Potential Imaging Workflow
Experimental design
Discuss the experimental protocol and determine the experimental steps and reagents.
Cell culture
Select appropriate media to maintain or culture cells under optimal conditions.
Cell labeling
Select the appropriate mitochondrial membrane potential dye probe (e.g., JC-1 dye) according to the experimental requirements and add the dye probe to the culture medium.
Signal optimization
Use reagents that reduce extracellular fluorescence and increase the photostability of the fluorescent dye to optimize the signal-to-noise ratio and reduce extracellular background fluorescence.
Imaging observation
Set the desired image acquisition parameters and acquire cell images.
Data analysis
Use the software to analyze and process the acquired image data, extract the fluorescence intensity data of mitochondrial membrane potential, and generate the corresponding graphs and curves.
Our Advantages
- Real-time recording of mitochondrial membrane potential captures rapid changes in mitochondrial membrane potential.
- Mitochondrial membrane potential can be analyzed on a large scale.
- Requires no intrusive manipulation of cells or use of toxic dyes.
- In addition to mitochondrial membrane potential, other cellular parameters such as cell morphology, cell proliferation, and apoptosis can be recorded and analyzed simultaneously.
Deliverables
- MMP imaging fluorescence images
- MMP quantitative data graphs
CD BioSciences has a professional team and advanced imaging equipment. The whole process of mitochondrial membrane potential imaging service is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.
- Zorova LD, et al. Mitochondrial membrane potential. Analytical Biochemistry, 2018, 552:50-59.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!