Yeast is a single-celled eukaryotic fungus with a diameter of 3-8 microns, which reproduces asexually through budding or division. During budding, the morphology of yeast cells changes. The optical microscope can directly observe this morphological change of a single cell in the cell suspension. Saccharomyces cerevisiae is the most commonly used strain in scientific research, baking and fermentation, and it plays a key role in the production of staple foods, medicines, and biofuels. Therefore, it is very important to monitor the growth of yeast cells by analyzing the dynamic images of yeast cells under various conditions in real time.

Figure 1. Asexual reproduction of yeast cell.

Monitoring Budding Yeast in Real Time

Flow cytometry is often used to characterize yeast populations. It can provide information such as the morphological characteristics and DNA content measurement of yeast, but it has not been widely used to monitor the growth process of yeast cells. In addition, imaging microscope or shake flask culture image cell counting is also a common method to assess the morphology of yeast cells, but it requires cell staining, which is cytotoxic. The in-situ microscope can be operated under various real-time conditions in in-situ experiments, and has been successfully applied to monitor the growth and budding activities of yeast in batch culture in real time.

CD BioSciences has been committed to the development of imaging technology for many years. We can monitor the growth and budding activities of yeast in batch culture in real-time through in-situ microscopes, identify and classify budding and non-budding yeast cells, and monitor the proportion of mature cells by budding index.

The Workflow of Monitoring Budding Yeast

The following are the specific steps of monitoring budding yeast in real time:

Delivery

Our Advantages

• Dynamic high resolution imaging
• Flexibility for sample imaging
• Quantify heterogeneity among budding yeast cell populations
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of monitoring budding yeast in real time is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Marbà-Ardébol, Anna-Maria, et al. "Real-time monitoring of the budding index in Saccharomyces cerevisiae batch cultivations with in situ microscopy." Microbial cell factories 17.1 (2018): 1-12.
2. Versari, Cristian, et al. "Long-term tracking of budding yeast cells in brightfield microscopy: CellStar and the Evaluation Platform." Journal of The Royal Society Interface 14.127 (2017): 20160705.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!