Neurite outgrowth is a widely used assay to study neuronal development and degeneration in vitro, which is of great significance in various research fields such as neuropathological diseases, neuronal injury/regeneration, and neuropharmacological research and screening. Imaging techniques can visualize and quantify neurite outgrowth and branching patterns, providing important information on the dynamics and characteristics of neuronal development. By imaging neurite outgrowth, researchers can measure parameters such as total length, number of neurites, and branches per cell, and evaluate the effects of potential therapeutic interventions. In addition, imaging can also help identify and evaluate potential therapeutic targets and compounds for neurological diseases.

Fig. 1 Image comparison for neurite growth analysis at ×10 (left) and ×20 (right) magnification (Dravid A., et al. 2021).

Neurite Outgrowth Imaging Services

CD BioSciences provides clients with a continuous, non-invasive neurite outgrowth imaging service that visually identifies biological processes of change and generates important kinetic data. Our services include, but are not limited to:

Neurite length analysis

CD BioSciences can perform analyses using client-selected neuron samples to quantify the maximum and average length of neurons and assess the stability of neural networks over time.

Neurotoxicity screening and neurodegeneration pathway studies

• Help clients screen for neurotoxicity.
• Study neurodegenerative pathways and neuroprotective therapies.
• Real-time measurement of neuronal differentiation processes.

Signaling mechanism assessment

• Evaluate signaling mechanisms that promote neuronal protrusion.
• Comprehensively interpret neurotoxicity response and compound screening data.
• Study factors that influence neural development and regeneration.

Sholl analysis

Using Sholl analysis to assess neuronal morphology and complexity, this technique allows us to:

• Study the effects of drug treatment on synapse development by measuring the maximum number of crossings of neuronal dendritic branches.
• Understand the efficacy of drug candidates by looking at the maximum number of synaptic crossings.

Neurite outgrowth assessment

Assessment of neurite outgrowth involves measuring various parameters of each cell to obtain information about the extent and characteristics of neurite growth under different experimental conditions or drug treatments. Parameters we measured include:

• The mean total length of protrusions and branches per cell.
• The mean number of processes per cell.
• The mean number of processes and branches per cell.
• The mean number of branches per cell.

Neurite Outgrowth Imaging Workflow

Our Advantages

• Enables continuous measurement data and images of neuronal synapse dynamics.
• Maximize your neuroscience research with the ability to image and analyze each well of a well plate.
• Exclusive reagents fluorescently label neurites, protecting your cells and not interfering with cell health or morphology.

Deliverables

• Neurite outgrowth raw images
• Neurite outgrowth quantitative data report

CD BioSciences has a professional team and advanced imaging equipment. The whole process of neurite outgrowth imaging service is operated by experienced technicians to ensure the accuracy of the experiment. We can also customize neurite growth imaging services for you. If you have any needs, please feel free to contact us.

1. Dravid A., et al. Optimised techniques for high-throughput screening of differentiated SH-SY5Y cells and application for neurite outgrowth assays. Sci Rep 11, 2021, 23935.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!