NHS Fluorescent Dye
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Catalog NO: VIR030204
Size: 1 mg; 5 mg
Subtype: 645 NHS Fluorescent Dye;
680 NHS Fluorescent Dye;
800 NHS Fluorescent Dye
680 NHS Fluorescent Dye;
800 NHS Fluorescent Dye
Introduction:Label antibodies, small molecules, proteins, or peptides with NIR fluorescent dyes. Fluorescent dyes for labeling antibodies, small molecules, proteins, or peptides are designed specifically to enable customized development of novel superbright fluorescent imaging agents. Dye properties, such as excellent brightness, hydrolytic stability, and photostability, as well as selection for minimal interaction with biological tissue for no potential loss of specificity, have been optimized and validated for use in in vivo or in vitro imaging. Available as NHS esters or maleimide reactive dyes for conjugation to either free amine (-NH3) or free thiol (-SH) containing molecules. Conjugate an optimal 1-3 dyes onto your protein or choose self-quenching dyes to make your own custom fluorogenic probes.
Choose the right fluorescent dye for your study:Suitable for labeling free amines (typically lysine residues): 645 NHS (Abs: 643 nm/Em: 660 nm); 680 NHS (Abs: 665 nm/Em: 688 nm); 800 NHS (Abs: 785 nm/Em: 810 nm)
Suitable for labeling free thiols (typically cysteine residues): 645 MAL (Abs: 643 nm/Em: 660 nm); 680 MA (Abs: 665 nm/Em: 688 nm)
SELF-QUENCHING DYES: 680 NHS (Abs: 673 nm/Em: 691 nm); 750 NHS (Abs: 750 nm/Em: 775 nm); 750 MAL (Abs: 750 nm/Em: 775 nm)
When a protein is labeled with multiple Self-Quenching Dye molecules, there is a shift in the absorbance of the dye, which will reduce the signal. Only choose Self-Quenching Dyes if your labeling ratio of dye: protein will be 1:1. If multiple dyes are to be conjugated onto your protein, a non-self-quenching Dye should be used. NHS dyes are suitable for labeling free amines (typically lysine residues), while MAL dyes are suitable for labeling free thiols (typically cysteine residues).
Suitable for labeling free thiols (typically cysteine residues): 645 MAL (Abs: 643 nm/Em: 660 nm); 680 MA (Abs: 665 nm/Em: 688 nm)
SELF-QUENCHING DYES: 680 NHS (Abs: 673 nm/Em: 691 nm); 750 NHS (Abs: 750 nm/Em: 775 nm); 750 MAL (Abs: 750 nm/Em: 775 nm)
When a protein is labeled with multiple Self-Quenching Dye molecules, there is a shift in the absorbance of the dye, which will reduce the signal. Only choose Self-Quenching Dyes if your labeling ratio of dye: protein will be 1:1. If multiple dyes are to be conjugated onto your protein, a non-self-quenching Dye should be used. NHS dyes are suitable for labeling free amines (typically lysine residues), while MAL dyes are suitable for labeling free thiols (typically cysteine residues).
Wavelength:645 nm; 680 nm; 800 nm
Category:Fluorescent Dyes & Cell Labeling Dyes
Shipping Condition:Blue Ice
Optical Imaging Classification:Fluorescence Imaging
Intended Use:For research use only. Not for use in diagnostic procedures.