The nuclear receptor (NR) is a lipid-soluble ligand-dependent transcription factor that regulates the transcription of target genes through the activation of endogenous or exogenous substances, and is involved in the regulation of a variety of physiological functions. NRs are widely distributed in the body and regulate a wide range of drug metabolizing enzymes and transporters, which can have a significant impact on the absorption, distribution, metabolism and excretion of drugs in the body. Most of the target genes regulated by nuclear receptors are involved in the formation of tumor multidrug resistance, which may become a new drug target for reversing tumor multidrug resistance. Therefore, the imaging analysis of nuclear receptors is important for the development of nuclear receptor-related drugs.

Figure 1. Structure of the human androgen receptor.

Nuclear Receptors Imaging Analysis

The development of live cell imaging techniques has facilitated the study of nuclear receptor localization and mobility. Based on fluorescent proteins, researchers can monitor the behavior of nuclear receptors in their physiological environment directly in living cells. In addition, fluorescence recovery (FRAP), fluorescence correlation spectroscopy (FCS) and fluorescence resonance energy transfer (FRET) can be used to study nuclear receptor interactions and their dynamics.

CD BioSciences has been committed to the development of imaging technology for many years. We can use a fluorescence microscopy technique with single molecule sensitivity (fluorescence correlation spectroscopy) to study the regulation of ligand binding by nuclear receptors in living cells. In addition, we can also study the localization and mobility of nuclear receptors.

Nuclear Receptors Imaging Analysis Workflow

The following are the specific steps of nuclear receptors imaging analysis

Delivery

Assess key properties of nuclear receptors for each fusion protein
High-quality confocal microscopy images of nuclear receptors
Dependence of nuclear receptor complexes on ligand type and dose and mode of activation
Factors affecting nuclear receptor migration
Other data that you need

Our Advantages

• Confocal microscopy images show clear nuclear receptor localization
• Visual analysis of nuclear receptors in living cells
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of nuclear receptors imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Brazda P, Szekeres T, Bravics B, et al. Live-cell fluorescence correlation spectroscopy dissects the role of coregulator exchange and chromatin binding in retinoic acid receptor mobility[J]. Journal of Cell Science, 2011, 124(21): 3631-3642.
2. Feige, Jérôme N., et al. Fluorescence imaging reveals the nuclear behavior of peroxisome proliferator-activated receptor/retinoid X receptor heterodimers in the absence and presence of ligand[J]. Journal of Biological Chemistry 280.18 (2005): 17880-17890.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!