Organoids Culture
Organoids are micro-organs that can be cultured by human adult stem cells (ASC) or pluripotent stem cells (PSC) in the laboratory, aiming to simulate the complex structure and function of human organs (such as lung, liver, or brain). Organoids usually contain co-cultures of cells and show a high degree of self-assembly. Compared with traditional two-dimensional (2D) cell cultures, organoids can better display complex cell responses and interactions in vivo. Therefore, it becomes a powerful tool for cancer research, neurobiology, stem cell research and drug screening.
Figure 1．Organoids for biomedical discovery and regenerative therapy (Bock C, et al. 2021).
Organoids Culture
As organoids and other 3D cell systems become increasingly complex, 3D imaging and analysis techniques need to be used to quantitatively describe organoids, so as to study the effects of disease phenotypes and compounds. CD BioSciences can cultivate different organoids through a variety of 3D culture technologies, and quantitatively analyze organoids with automatic confocal imaging system and 3D imaging analysis software to obtain the best results.
| Organoid Type | Gastrointestinal organoids, neural organoids, liver organoids, prostate organoids, kidney organoids, lung organoids |
| Culture Method | Scaffold-Based Technologies: natural hydrogel, synthetic hydrogel, customized method according to your needs Non-Scaffold-Based Technologies: magnetic levitation, hanging drop microplates, customized method according to your needs |
Organoid Culture Workflow
Organoids can be used as a valuable tool for translational research, including the development of personalized medicines, where in vitro model culture from individual human subjects can be used to develop customized treatment plans for each patient. The following is the general process of organoid culture:
Step 1
2D pre-culture
Primary cells (i.e. intestinal, lung or kidney cells) or induced pluripotent stem cells can differentiate and self-assemble into various tissue-specific organoids.
Step 2
Development of 3D organoids
Pre-mix the cells and Matrigel at room temperature and add drops to the 24-well plate. The plate is then placed in an incubator to form a solid drop dome. Then add culture medium and incubate for 7 days or longer to promote cell growth and differentiation into specific tissues.
Step 3
Organoid culture
Organoid culture is a long process, which may include different steps and use of different media. In this process, timely imaging is needed to monitor cell phenotypes for understanding developmental biology.
Step 4
Monitoring and reading
Before conducting experiments, it is necessary to monitor organoids and describe their characteristics to ensure that they have proper tissue structure and differentiation. High-content imaging allows monitoring and observing the growth and differentiation of organoids.
Step 5
Confocal imaging and 3D analysis
Confocal imaging and 3D analysis of organoids can observe and quantify particle size distribution, cell number, and quantify cell proliferation and the expression of specific markers. A variety of quantitative descriptions of organoids can be used to study disease phenotypes and compound effects.
Delivery
Organoid size distribution
Organoid volume
Number of cells
Cell content
Cell viability
Cell Proliferation
Expression of specific markers
Other relevant data
Our Advantages
- Confocal imaging and 3D image analysis
- Provide three-dimensional information of cells
- Provide various types of organoids
- Experienced scientists provide experimental consultation
- Reasonable price and short turnaround time
CD BioSciences has technicians with rich working experience in the field of 3D cell culture. We can design a personalized 3D cell culture program for you to obtain organoids that meet your needs, and we also provide you with organoid quantitative analysis reports. If you have any needs, please feel free to contact us.
- Bock C, Boutros M, Camp J G, et al. The organoid cell atlas[J]. Nature Biotechnology, 2021, 39(1): 13-17.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!