Phagocytosis plays an important role in the immune system in removing pathogens and renewing cells to resolve inflammatory flare-ups, prevent chronic inflammatory or autoimmune diseases, and remove apoptotic tumor cells after treatment with anticancer drugs. Live cell imaging technology changes the traditional manual processing method of analyzing phagocytosis, providing a real-time visualization and quantitative assessment technology. Phagocytosis live cell imaging allows for easy quantification of phagocytosis kinetics, screening and characterization of compounds that modulate phagocytosis, thereby enhancing our understanding of immune function and disease processes.

Fig. 1 Phagocytosis of Macrophage.

Phagocytosis Imaging Services

CD BioSciences has access to real-time live cell imaging equipment to provide more comprehensive real-time phagocytosis imaging services. Our services include, but are not limited to:

Real-time quantification of microbial killing

Able to effectively quantify the rate and level of phagocytosis of pathogens such as Gram-negative or Gram-positive bacteria, visualizing and deeply analyzing in real time which microorganisms are killed by phagocytosis.

Track phagocytosis using fluorescent reagents

By using fluorescent reagents, such as pH-sensitive red fluorescent reagents, we can quantify phagocytosis rates based on the amount of fluorescent signaling in an interference-free and real-time manner, which provides insights into receptor-ligand interactions as well as the effects of pathway modulators on phagocytic mechanisms.

Real-time visualization and quantification of phagocytosis kinetics

We can track and measure the progression of phagocytosis at multiple time points, generating images plotted as a function of time and quantifying the process of phagocytosis kinetics.

Assessing drug dose-dependent phagocytosis inhibition

Treat cells with different drug concentrations to assess the dose-response relationship between drug concentration and degree of phagocytic inhibition. This helped to determine the dose-dependent inhibition of phagocytosis by the drug and calculate IC50 (half inhibitory concentration) values.

Quantitative analysis of antibody-mediated cell phagocytosis

• Enable quantitative measurement of phagocytosis of diseased, dead or dying cells using phagocytes and target cells of the customer's choice.
• Study antibody-mediated phagocytosis or cellular phagocytosis, such as phagocytosis of apoptotic cells or anti-CD47 antibody-mediated phagocytosis.

Phagocytosis Imaging Workflow

Our Advantages

• Time efficiency. Analyze phagocytosis faster and more efficiently by reducing operating time by eliminating tedious steps such as repairs or quenching.
• High quality data. We provide quantitative and reproducible data to ensure the accuracy and consistency of the results obtained.
• Continuous monitoring. Providing high sensitivity, accuracy and throughput, we can continuously monitor phagocytosis over a long period of time (from hours to days), providing a comprehensive understanding of the dynamics of the phagocytic process.
• Assessment of chemical effects. Our services provide a detailed understanding of the enhancement or inhibition of phagocytic activity by chemicals and compounds.

CD BioSciences has a dedicated team and state-of-the-art imaging equipment to quantify cellular phagocytosis throughout the time duration of the assay. If you have any needs, please feel free to contact us. We can also customize phagocytosis imaging services for you according to specific needs.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!