Prostanoid receptors belong to the lipid receptor family in the G protein-coupled receptor superfamily. They play extremely important biological functions in the human body and mediate important physiological activities such as cell proliferation, differentiation and apoptosis. Prostanoids in humans include the prostaglandin hormones PGD2, PGE2, PGF2α, PGI2, and thromboxane A2, which function by interacting with their respective prostanoid receptors. Among them, thromboxane A2 receptor TP is an important target of drugs for the treatment of cardiovascular diseases such as atherosclerosis and hypertension. Therefore, imaging analysis of the mechanism of action of prostanoid receptors and their ligand molecules is very important.

Figure 1. Schematic diagram of the three-dimensional structure of the thromboxane A2 receptor TP (Hengxin, et al. 2019).

Prostanoid Receptors Imaging Analysis

The mechanism of action of prostanoids and their ligand molecules is unclear, which makes the development of anti-cardiovascular disease drugs relatively slow, and candidate drug molecules generally have problems such as poor specificity and strong side effects. In recent years, researchers have used various imaging techniques to study the structure of prostanoid receptors and the mechanism of their interaction with ligands, which provides an important basis for the development of drugs for the treatment of cardiovascular diseases.

CD BioSciences has been committed to the development of imaging technology for many years. We can use confocal laser scanning microscopy and fluorescence correlation spectroscopy to study the structure and kinetics of prostanoid receptor complexes with their ligands in living cells. This provides a high-precision structural template for drug design targeting prostanoid receptors, and has a very important guiding significance for the development of new drugs with high selectivity.

Prostanoid Receptors Imaging Analysis Workflow

The following are the specific steps of prostanoid receptors imaging analysis

Delivery

High-quality confocal microscopy images of prostanoid receptors
The kinetics of prostanoid receptor interaction with their ligands
Other data that you need

Our Advantages

• Confocal microscopy images show clear prostanoid receptor localization
• Visual analysis of prostanoid receptors in living cells
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of prostanoid receptors imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Hengxin, Fan, Shuanghong, et al. Structural basis for ligand recognition of the human thromboxane A2 receptor[J]. Nature Chemical Biology, 2019, 15(1):27-33.
2. Woodward D F, Wang J W, Ni M, et al. In vivo studies validating multitargeting of prostanoid receptors for achieving superior anti-inflammatory effects[J]. The FASEB Journal, 2017, 31(1).
3. Guo H, An S, Ward R, et al. Methods used to study the oligomeric structure of G-protein-coupled receptors[J]. Bioscience Reports, 2017, 37(2).

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!