The role of the soil microbial community is to decompose and absorb the organic matter in the soil and aggregate soil particles. A healthy soil microbiome relies on various bacterial strains, each of which plays a unique role in maintaining soil balance. Bacterial viability is an effective indicator for evaluating the status of soil microbes in different environments. Bacillus is a kind of bacteria commonly found in surface soil, so imaging and analysis of its viability is very important to assess the balance of soil microbiota.

Figure 1. Arrangement of Bacilli Bacteria (shape of bacillus bacteria).

Quantitative Image Analysis of Bacilli Viability in Soil Samples

The tilting plate method is a commonly used method for detecting the viability of bacteria, which can directly count the microbial cultures prepared from the sample colony suspension. However, this method takes a long time and cannot directly observe the morphology of bacteria. In recent years, with the development of fluorescent staining and microscopy, it is possible to visually analyze the morphology and viability of bacteria.

CD BioSciences has been committed to the development of fluorescent dye and imaging technology for many years. We use a microscope to image and analyze the viability of the bacilli isolated from the soil sample, which can provide you with the number of living cells and distinguish the staining objects in the sample by morphological indicators.

The Workflow of Quantitative Image Analysis of Bacilli Viability in Soil Samples

The following are the specific steps of quantitative image analysis of bacilli viability in soil samples:

Delivery

Our Advantages

• Maintain data integrity
• Reliable and reproducible results
• Flexibility for sample imaging
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of quantitative image analysis of bacilli viability in soil samples is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

1. Laflamme, Christian, et al. "Assessment of bacterial endospore viability with fluorescent dyes." Journal of Applied Microbiology 96.4 (2004): 684-692.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!