RNA Structure Imaging Services
RNA is usually transcribed from DNA, which is a bridge for DNA expression to produce proteins, and plays a vital role in a variety of cellular activities. Because RNA sequence is conservative, its function mainly depends on the secondary structure of RNA. The secondary structure of RNA is a double-stranded structure formed according to the principle of base complementary pairing. In addition, RNA can fold into high-level structures and form dynamic complexes with effector proteins. Visual analysis of these 3D structures of RNA is still challenging. Therefore, it is necessary to develop effective imaging techniques to study RNA folding and its structure.
Figure 1. 3D image of RNA macromolecule skeletal formula.
RNA Structure Imaging Analysis
RNA-protein interaction is the basis of RNA molecular structure and function. Due to its strong adaptability and concise experiment, chemical detection has become a widely applicable method for analyzing RNA structure and RNA-protein interaction. However, chemical detection cannot visualize and characterize RNA structure and RNA-protein interactions in living cells.
CD BioSciences has been committed to the research and development of fluorescent probes and imaging technology for many years. We can image and analyze the structure of different types of RNA and the interaction between proteins and different types of RNA in cells by RNA fluorescent in situ hybridization.
RNA Structure Imaging Analysis Workflow
The following are the specific steps of RNA structure imaging analysis:
Step 1
Fill in the online form to submit your requirements for RNA structure imaging analysis.
Step 2
Send us the RNA that need to be imaged and analyzed.
Step 3
We image and analyze the structure of RNA and the interaction between proteins RNA by RNA fluorescent in situ hybridization.
Step 4
After the experiment is completed, we will send the sample and experimental data to you.
Delivery
Characterization data of different types of RNA structures
Images and analysis data of the interaction between proteins and different types of RNA
Provide other data according to your needs
Our Advantages
- High-resolution imaging of RNA structure and location
- Minimize background noise
- Experienced scientists provide experimental consultation
- Reasonable price and short turnaround time
CD BioSciences has a professional team and advanced imaging equipment. The entire process of RNA structure imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.
- Mustoe, Anthony M., et al. "RNA base-pairing complexity in living cells visualized by correlated chemical probing." Proceedings of the National Academy of Sciences 116.49 (2019): 24574-24582.
- Duan, Ningjun, et al. "Visualization and characterization of RNA–protein interactions in living cells." Nucleic acids research 49.18 (2021): e107-e107.
- Chen, Fei, et al. "Nanoscale imaging of RNA with expansion microscopy." Nature methods 13.8 (2016): 679-684.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!