Stem cells can produce various specialized cells through mitosis and differentiation, which are of great significance for regenerative medicine, pathological analysis, and cancer grading and diagnosis. However, accurate monitoring of cell mitotic activity is challenging due to the spatial dispersion of cells and the spatiotemporal stochasticity of mitotic activity. Therefore, high-resolution imaging technology has become an important tool for studying stem cell mitosis.

Fig. 1 Live cell imaging before mitosis into NEBD (Liang H., et al. 2021).

Services Overview

Using high-resolution live cell imaging technology, CD BioSciences can overcome the limitations caused by spatial dispersion of cells and randomness of mitosis, enabling accurate and detailed monitoring of the stem cell division process.

Stem Cell Mitosis Imaging Services Content

CD BioSciences offers high-resolution live cell imaging services that allow for the examination of the stem cell cycle at the single-cell level. Our advanced imaging equipment eliminates the need for expensive fluorescent antibodies and complex processing protocols, providing detailed information about the cell cycle.

Visualization of stem cell mitosis stages

CD BioSciences can use high-resolution live-cell imaging to visualize different stages of stem cell mitosis, including:

• Prophase (A)
• Prometaphase (B)
• Metaphase (C)
• Anaphase (D)
• Telophase (E)
• Cytokinesis (F)

Provides information about the stem cell cycle and allows precise observation of each stage of mitosis.

Detailed information about key stem cell mitotic events

Our stem cell mitosis imaging service can capture events such as:

• Prophase

Captures the process of chromatin condensing into chromosomes.

• Metaphase

Record the arrangement of chromosomes along the mid-phase plate.

• Telophase

Observe the division and reorganization of the nuclear membrane.

Characterization and analysis of stem cell function

• Accurate identification of the mitotic stage that the stem cell is in.
• Insight into the mechanisms of DNA damage and repair in stem cells.
• Valuable data on the process of stem cell regeneration.

Stem Cell Mitosis Imaging Workflow

Our Advantages

• Enable observation of live cells without destroying the sample and using expensive fluorescent antibodies, enabling stem cells to maintain their ability to self-renew and differentiate.
• Allow observation of the details of stem cell mitosis and changes, capturing high-detail images of cells.
• Allow access to three-dimensional structures of stem cells in mitosis and enables continuous observation of the process of stem cell mitosis.

CD BioSciences has a professional team and advanced imaging equipment to provide customers with accurate and reliable stem cell mitosis high-resolution imaging. Help clients deeply understand the mitotic process and cell cycle of stem cells. If you have any needs, please feel free to contact us.

1. Liang H., Esposito A., De S. et al. Homeostatic control of polo-like kinase-1 engenders non-genetic heterogeneity in G2 checkpoint fidelity and timing. Nat Commun 5, 2014, 4048.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!