Tumor Area Quantification
Molecular pathology is becoming more and more important in today's pathology. One of the main challenges of any molecular test is that it can reliably detect mutations in a sample composed of a mixture of tumor cells and normal cells, especially when the tumor content is very low. The percentage of tumor cells is usually estimated by a pathologist, which is important for the final diagnosis and prognosis of cancer.
Figure 1．Schematic and descriptive illustration of the different stages of a bladder tumor
Tumor Area Quantification
Microscopic examination of tissue morphology using H&E is a standard practice for visual analysis of tissue specimens in histopathology. The percentage of tumor cells is an important indicator to evaluate the total area of the tumor. CD BioSciences uses H&E to stain pathological tissues to distinguish tumor cells from non-tumor cells, then counts tumor cells and quantitatively evaluates tumor area.
| Analysis method | Brightfield imaging |
| Sample requirement | Whole tissue fixed and stored in PBS with 0.05% azide Formalin fixed paraffin embedded (FFPE) tissue blocks Tissues embedded in OCT Pre-stained and mounted slides Digitized slide images |
| Markers | Hematoxylin and eosin |
| Image analysis | Cell counting Tumor area quantification Analysis of nuclear morphology |
Tumor Area Quantification Workflow
The following is the general workflow of tumor area quantification:
Step 1
Sample preparation
Tissue samples are transferred to PBS w/0.05% azide or made into FFPE, OCT compounds.
Step 2
Sample staining
The samples were processed, sectioned and stained with hematoxylin and eosin.
Step 3
Imaging
The sample slide is imaged under a high-throughput slide scanner.
Step 4
Analyze
Analyze the images according to customer requirements.
Step 5
Send data
Send images and quantitative reports to customers.
Delivery
Whole slide images in RGB format
ROI masks (tumor and non-tumor regions)
Data tables containing cell counts
Other relevant data
Our Advantages
- A large number of tissue sections can be imaged and analyzed in a short time
- Provide reliable and accurate test results
- Experienced scientists provide experimental consultation
- Reasonable price and short turnaround time
CD BioSciences has researchers with rich experience in the field of pathological imaging analysis, who can skillfully use imaging equipment and image analysis software. We can provide you with tumor area quantification services by brightfield imaging. If you have any needs, please feel free to contact us.
- Lhermitte, Benoît, et al. "Adequately defining tumor cell proportion in tissue samples for molecular testing improves interobserver reproducibility of its assessment." Virchows Archiv 470.1 (2017): 21-27.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!