CD BioSciences offers a comprehensive suite of solutions for tumor immune microenvironment quantification analysis, including staining, imaging, and data analysis. Through our services, researchers can identify different types of immune cells in tumor tissues using multiple markers. We are able to demonstrate the distribution and number of immune cells in tissue in situ in an imaging manner, thus providing important spatial distribution information about the tumor microenvironment. This spatial distribution information helps to accurately understand the roles and functions of immune cells within a tumor or in the tumor microenvironment, and drives research and innovation in tumor immunotherapy.

Fig. 1 3D illustration of the tumor microenvironment showing that the microenvironment will influence tumor growth and spread.

Tumor Immune Microenvironment Quantification Analysis Services

Tumor infiltrating immune cell analysis services

CD BioSciences will provide tumor-infiltrating immune cell analysis services to support the study of tumor infiltrating immune cells in homogeneous tumor models and to assist in evaluating the efficacy of novel immunotherapeutic agents. Homogeneous tumor models are widely used in immuno-oncology because they have an intact and fully functional immune system and can recapitulate the complex interactions between the immune system and the tumor. With these models, we can help researchers monitor changes in the immune system throughout the body or at the tumor site and evaluate the response to immunotherapies used in combination or as monotherapy.

Quantitative analysis services of immune cell infiltration in tumor tissue

We provide services for the identification and quantitative analysis of tumor infiltrating lymphocytes (TIL) to support the evaluation of novel immunotherapeutic agents. We can evaluate the following parameters/variables to help clients determine the mechanism of action of immunotherapies:

➢ Inflammatory states in tumor models
➢ Quantification of treatment-related effector cells in tumor models
➢ Activation and depletion status of immune cells
➢ Host immune system and cytokine response
➢ Degree of immune cell infiltration at the tumor site
➢ Off-target/toxic effects

Our analytical methods for tumor infiltrating immune cells

For the analysis of tumor-infiltrating immune cells, we will mainly provide the following techniques and methods to help our clients perform quantitative analysis and evaluation, include:

➢ Immunohistochemistry (IHC) technology
Spatial information such as location and distribution of immune cells is visualized by staining tissue sections and using imaging techniques. This is a semi-quantitative immunoassay assay that provides information about the density, degree of infiltration and subpopulation distribution of immune cells within the tumor tissue.
➢ Immunofluorescence (IF) technology
Using fluorescently labeled antibodies to visualize specific immune cells or molecules, IF has greater sensitivity than IHC and the ability to perform multi-parameter analyses.
➢ Multiple spatial tissue analysis (MSTA) methods
MSTA provides information on the distribution and relative position of markers or cells within a tissue sample. Its ability to evaluate hundreds of nucleic acid and/or protein markers simultaneously in a spatially correlated environment helps us to better understand the distribution patterns of immune cells and the interaction between them and the tumor microenvironment.

Tumor microenvironment quantitative pathology imaging analysis services

CD BioSciences provides quantitative pathology imaging services for tumor microenvironment using advanced imaging technology and intelligent tissue image analysis. We can support fluorescence imaging by meeting the imaging needs of various general tissue staining samples. Meanwhile, we will work with multiple reagents for in situ multi-marker analysis of tissues to provide comprehensive quantitative information on the tumor microenvironment for our clients. Our services include but are not limited to:

FFPE tissue section analysis service

Perform tumor immunology studies on fixed-embedded tissue sections, which allows us to assist our clients with in-depth observation, quantification, statistics, and phenotypic identification of the tumor microenvironment.

Multispectral imaging analysis service

Using multispectral imaging technology, we can simultaneously analyze multiple markers, providing more comprehensive information that reveals the overall and spatially correlated distribution of multiple immune cell subpopulations, molecular markers, or intercellularity.

CD BioSciences has a professional team and advanced imaging equipment. The whole process of tumor immune microenvironment quantification analysis services is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!