Vascular morphology imaging is an important technique used to characterize tissue substructure. Traditional histological sections (microtome) have limitations in characterizing vascular tissue sub-structures, such as problems with tearing, compression, and folding. CD BioSciences utilizes non-destructive 3D imaging for accurate submicron structural characterization of vascular tissues and for quantitative analysis of vascular tissues. Our services help researchers to explore in depth the relationship between vascular tissue and disease, thereby advancing the understanding of disease mechanisms.

Fig. 1 Blood vessels under microscope.

Services Overview

CD BioSciences provides vascular tissue morphology imaging services utilizing advanced imaging technologies. Our services are used in various fields, including central nervous system vascular morphology imaging, cardiac vascular morphology imaging, pulmonary vascular morphology imaging, liver vascular morphology imaging, renal vascular morphology imaging, and skeletal vascular morphology imaging. Our services help our clients to study the relationship between various diseases and vascular tissue morphology, providing an in-depth understanding of the pathophysiological changes of diseases.

Vascular tissue morphology microCT 3D imaging analysis services

CD BioSciences utilizes micro-CT (micro-CT) technology to provide detailed 3D imaging and analysis of small animal blood vessels, helping clients achieve non-destructive in vivo observation of vascular morphology and qualitative and quantitative analyses. Our technology possesses the following advantages: ultra-high spatial resolution, high-level geometric magnification, fast imaging speed, flat-panel detector with many pixels and small voxels, micron-level spatial resolution, and detailed 3D imaging analysis. Our services include but are not limited to:

Vascular structure and morphometric characterization

• Utilize MicroCT technology for vascular imaging to preserve arterial structure and measure new morphological features.
• Resolve details of elastic fibers, collagen protofiber bundles, and vascular smooth muscle cell structures in the medial lamellar unit of the vessel, showing structural heterogeneity in the medial interlamellar region of the vasculature.
• Study vascular tissue structure at multiple length scales and label regions of interest, enabling the study of tissue structure at different scales.

3D image analysis and segmentation

• CD BioSciences employs semi-automated image analysis to segment low-contrast but structurally and compositionally distinct regions of vascular tissue.
• Quantitative characterization of 3D tissue structures provides insight into morphological changes and morphometric parameters, and assesses the effect of pressure on vessel wall and interface topography.
• Obtain information on the geometry and spatial distribution of the vasculature, endothelial and epithelial layers.

Morphological parameter measurements

• Rapidly characterize morphological parameters at all points around a virtual vessel cross-section to avoid sampling errors and gain insight into changes in vessel tissue morphology.
• Analyze morphological parameters such as lumen, cross-sectional area, and layer thickness of vessel cross-sections to assess quantitative changes in vascular remodeling and morphological characteristics.
• Compare vessel morphology under different pressure conditions to understand the effects of physiological and dynamic loading on vascular tissue.

Fig. 2 Imaging of retinal blood vessels in normal eyes.

Quantitative imaging analysis of vascular tissue and vascular network

CD BioSciences uses tissue transparency technology and confocal microscopy imaging technology to study the entire vascular tissue area and visualize the complete vasculature at 10X, 20X, and 40X magnification. We provide customized quantitative analysis data based on clients needs to help evaluate and quantify vascular tissue/network. Our constant parameters mainly include:

• Blood vessel area/volume
• Branch length and frequency
• Vessel tortuosity
• Vessel orientation

Deliverables

• Blood vessel raw image
• Vascular quantitative data table and histogram
• Statistical analysis data report

CD BioSciences has a professional team and advanced imaging equipment. The whole process of vascular tissue morphology  imaging analysis services is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!