Viruses as an intracellular parasite whose life activities are closely related to host cells. Virus-host cell interactions are an essential part of the life cycle of any virus, as all viruses reproduce within the host cell. Visual analysis of virus-host cell interactions not only helps researchers to understand the mechanisms by which viruses infect cells and cause disease, but also provides important targets for the development of antiviral drugs.

Figure 1. Imaging techniques used to study virus-host interactions during the viral replication cycle (McClelland R D, et al. 2021).

Virus-Host Cell Interactions Imaging Analysis

The diameter of many virus particles is usually between tens to hundreds of nanometers smaller than the wavelength of visible light, and traditional optical microscopes are limited by the diffraction limit and cannot directly observe them. In recent years, transmission electron microscopy (TEM) has been used for imaging analysis of virus particles. However, sample preparation for TEM is laborious, which limits its application in the field of virology. Compared to other imaging methods, imaging flow cytometry and confocal immunofluorescence microscopy are practical and rapid visualization methods for studying virus-host cell interactions.

CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We can use imaging flow cytometry and confocal immunofluorescence microscopy to study virus-host cell interactions to understand the mechanism of virus infection of host cells and to find new targets for antiviral drugs.

Virus-Host Cell Interactions Imaging Analysis Workflow

The following are the specific steps of virus-host cell interactions imaging analysis

Delivery

Image of virus-host cell interactions
Virus-receptor colocalization
Host receptor localization
Characterize viral replication complexes
ldentify viral restriction factors
Virion assembly and viral egress
Changes in virus and host protein colocalization over time and under different conditions
Other data that you need

Our Advantages

• The generated images are informative
• Track intracellular distribution and localization of viral proteins or nucleic acids
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of virus-host cell interactions imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

1. McClelland R D, Culp T N, Marchant D J. Imaging flow cytometry and confocal immunofluorescence microscopy of virus-host cell interactions[J]. Frontiers in Cellular and Infection Microbiology, 2021, 11.
2. Quemin E R J, Machala E A, Vollmer B, et al. Cellular electron cryo-tomography to study virus-host interactions[J]. Annual Review of Virology, 2020, 7: 239-262.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!